The LIQUIDPlexTM ctDNA 28 Kit is an advanced and user-friendly solution for targeted next generation sequencing (NGS) of circulating cell-free tumor DNA (ccfDNA/cfDNA/ctDNA) from 28 genes commonly associated with solid tumor type cancers.
Relevant Fragment Capture
Anchored Multiplex PCR (AMP™)-based target enrichment preferentially enriches for highly fragmented ctDNA over high molecular weight genomic DNA to reduce background and increase detection sensitivity of low-allele fraction (AF) mutations.
Single-day library prep
NGS-ready libraries can be prepared in under 8 hours with 2.5 hours of hands-on time, providing sequencing results in as little as 3 days.
Error correction & Quantitative Analysis
Molecular barcode sequences ligated prior to amplification enable duplicate read binning and advanced error correction for confident variant calls.
Sensitive & Specific Variant Detection
Reliably detect high AF (5%) variants using as little as 5ng total cfDNA input and low AF (1%) variants from as little as 10ng.
Solution for Illumina platforms. Access to Archer Analysis software for data analysis and interpretation.
Genes:
The LIQUIDPlexTM ctDNA 28 assay can be combined with the VariantPlex (DNA-based) and FusionPlex (RNA-based) products for a more comprehensive view of these genetic studies.
