Human Platelet Antigens (HPA) Fluogene typing

FluoGene is a unique method for molecular HPA-, RBC- (blood group) and HLA typing combining all advantages of the SSP–PCR with the speed of endpoint fluorescence detection.

The analysis is based on a specifically modified TaqMan® probe system by inno-train. There are two options for detection: the FluoVista analyzer or the FluoQube – inno-train’s real-time device.

• Up to four fluorophores are detected by the FluoVista® analyzer and an integrated data evaluation is realized by the FluoGene® software.
• FluoQube® combines the FluoGene® method with real-time PCR. This system allows amplification and fluorescence reading in one system. The real-time data are automatically imported into the FluoGene® Software for result evaluation.

Human Platelet Antigens (HPA) are glycoproteins expressed on the membrane of platelets. Antibodies against the Human Platelet Antigens can cause immune reactions in the recipient´s bood leading to the lysis of the transfused platelets.

HPA-Fluogene Kit  enables the detection of the two variants (a and b) of the platelet antigens HPA-1, HPA-2, HPA-3, HPA-4, HPA-5, HPA-6, HPA-9 and HPA-15 . The software indicates the alleles present in each sample.

All FluoGene kits are CE marked in accordance with the IVD Directive 98/79/EC  and manufactured and tested under a quality system in compliance with DIN EN ISO 13485.

ADVANTAGES

• PCR-SSP method without gel electrophoresis within 90 minutes
• no hybridization and washing steps
• display of typing results immediately after the post-read
• no risk of post-PCR contaminations
• low DNA consumption
• user-friendly software solution, fully-automated calculation of results
• same system for HLA-, RBC- and HPA
• use of patented fluorescent dyes and quencher