SCOPE™ Kits
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Twist Human Core Exome and Twist Human Core Exome + Ref Seq are the two capture solutions for human exome sequencing by Twist Bioscience.
The superior performance of the Twist Core Exome provides the optimal solution for sequencing human coding genes, while focusing on the most accurate curated subset of genes: that of the CCDS database. In addition, Twist offers the Human Core Exome + RefSeq option, which expands the content of the Twist Human Core Exome to provide coverage of greater than 99% of protein coding genes. The sequences covered have been selected from several updated databases (CCDS, RefSeq and GENCODE) to ensure complete and up-to-date content.
The Human Core Exome offers the potential to reduce sequencing costs of the Human Core Exome + RefSeq option by increasing the samples that can be sequenced in each run, and achieving a higher read depth with uncompromising data quality.
Key aspects:
The kits include the reagents for the preparation of individual libraries: by mechanical fragmentation or by enzymatic fragmentation, using Unique Dual Indices or Combinatory Dual Indices; and the reagents necessary for the enrichment of the regions of interest, by Standard or Fast hybridization, offering flexibility to the users, but without compromising the quality of the data.
The libraries obtained are compatible with Illumina's NGS platforms.
Customization
The content of the Twist Human Core Exome can be easily expanded with the Twist Custom Blended Panels option.
Reference | Product | Sample number |
---|---|---|
Panels: | ||
103077 | Twist Human Core Exome + RefSeq Panel, 2 reactions kit | 16 |
103078 | Twist Human Core Exome + RefSeq Panel, 12 reactions kit | 96 |
102025 | Twist Human Core Exome, 2 reactions kit | 16 |
102026 | Twist Human Core Exome, 12 reactions kit | 96 |
Library prep and enrichment reagents: | ||
Enzymatic fragmentation and CDIs (Combinatory Dual Index) | ||
101904 | Twist EF Library Prep + CDIs and Fast Hyb, 12 reactions | 96 |
104202 | Twist EF Library Prep with amp mix + CDIs and Fast Hyb with amp mix, 12 reactions | 96 |
101905 | Twist EF Library Prep + CDIs and Standard Hyb, 12 reactions | 96 |
104203 | Twist EF Library Prep with amp mix + CDIs and Standard Hyb with amp mix, 12 reactions | 96 |
Mechanical fragmentation and CDIs (Combinatory Dual Index) | ||
101906 | Twist MF Library Prep + CDIs and Fast Hyb, 12 reactions | 96 |
104204 | Twist MF Library Prep with amp mix + CDIs and Fast Hyb with amp mix, 12 reactions | 96 |
101907 | Twist MF Library Prep + CDIs and Standard Hyb, 12 reactions | 96 |
104205 | Twist MF Library Prep with amp mix + CDIs and Standard Hyb with amp mix, 12 reactions | 96 |
Enzymatic fragmentation and UDIs (Unique Dual Index | ||
101896 | Twist EF Library Prep + UDIs and Fast Hyb, 2 reactions | 16 |
104383 | Twist EF Library Prep 2.0 + UDIs and Fast Hyb, 2 reactions | 16 |
104182 | Twist EF Library Prep with amp mix + UDIs and Fast Hyb, 2 reactions | 16 |
101897 | Twist EF Library Prep + UDIs and Standard Hyb, 2 reactions | 16 |
104384 | Twist EF Library Prep 2.0 + UDIs and Standard Hyb, 2 reactions | 16 |
104183 | Twist EF Library Prep with amp mix + UDIs and Standard Hyb, 2 reactions | 16 |
101900 | Twist EF Library Prep + UDIs and Fast Hyb, 12 reactions | 96 |
104385 | Twist EF Library Prep 2.0 + UDIs Plate A and Fast Hyb with amp mix, 12 reactions | 96 |
104186 | Twist EF Library Prep with amp mix + UDIs and Fast Hyb with amp mix, 12 reactions | 96 |
101901 | Twist EF Library Prep + UDIs and Standard Hyb, 12 reactions | 96 |
104386 | Twist EF Library Prep 2.0 + UDIs Plate A and Standard Hyb with amp mix, 12 reactions | 96 |
104187 | Twist EF Library Prep with amp mix + UDIs and Standard Hyb with amp mix, 12 reactions | 96 |
103895 | Twist EF Library Prep + UDIs Plate B and Fast Hyb, 12 reactions | 96 |
104387 | Twist EF Library Prep 2.0 + UDIs Plate B and Fast Hyb with amp mix, 12 reactions | 96 |
104190 | Twist EF Library Prep with amp mix + UDIs Plate B and Fast Hyb with amp mix, 12 reactions | 96 |
103896 | Twist EF Library Prep + UDIs Plate B and Standard Hyb, 12 reactions | 96 |
104388 | Twist EF Library Prep 2.0 + UDIs Plate B and Standard Hyb with amp mix, 12 reactions | 96 |
104191 | Twist EF Library Prep with amp mix + UDIs Plate B and Standard Hyb with amp mix, 12 reactions | 96 |
103899 | Twist EF Library Prep + UDIs Plate C and Fast Hyb, 12 reactions | 96 |
104389 | Twist EF Library Prep 2.0 + UDIs Plate C and fast Hyb with amp mix, 12 reactions | 96 |
104194 | Twist EF Library Prep with amp mix + UDIs Plate C and Fast Hyb with amp mix, 12 reactions | 96 |
103900 | Twist EF Library Prep + UDIs Plate C and Standard Hyb, 12 reactions | 96 |
104390 | Twist EF Library Prep 2.0 + UDIs Plate C and Standard Hyb with amp mix, 12 reactions | 96 |
104195 | Twist EF Library Prep with amp mix + UDIs Plate C and Standard Hyb with amp mix, 12 reactions | 96 |
103903 | Twist EF Library Prep + UDIs Plate D and Fast Hyb, 12 reactions | 96 |
104391 | Twist EF Library Prep 2.0 + UDIs Plate D and Fast Hyb with amp mix, 12 reactions | 96 |
104198 | Twist EF Library Prep with amp mix + UDIs Plate D and Fast Hyb with amp mix, 12 reactions | 96 |
103904 | Twist EF Library Prep + UDIs Plate D and Standard Hyb, 12 reactions | 96 |
104391 | Twist EF Library Prep 2.0 + UDIs Plate D and Standard Hyb with amp mix, 12 reactions | 96 |
104199 | Twist EF Library Prep with amp mix + UDIs Plate D and Standard Hyb with amp mix, 12 reactions | 96 |
Mechanical fragmentation and UDIs (Unique Dual Index) | ||
101902 | Twist MF Library Prep + UDIs and Fast Hyb , 12 reactions | 96 |
104184 | Twist MF Library Prep with amp mix + UDIs and Fast Hyb, 2 reactions | 16 |
104188 | Twist MF Library Prep with amp mix + UDIs and Fast Hyb with amp mix, 12 reactions | 96 |
101903 | Twist MF Library Prep + UDIs and Standard Hyb, 12 reactions | 96 |
104185 | Twist MF Library Prep with amp mix + UDIs and Standard Hyb, 2 reactions | 16 |
104189 | Twist MF Library Prep with amp mix + UDIs and Standard Hyb with amp mix, 12 reactions | 96 |
103897 | Twist MF Library Prep + UDIs Plate B and Fast Hyb, 12 reactions | 96 |
104192 | Twist MF Library Prep with amp mix + UDIs Plate B and Fast Hyb with amp mix, 12 reactions | 96 |
103898 | Twist MF Library Prep + UDIs Plate B and Standard Hyb, 12 reactions | 96 |
104193 | Twist MF Library Prep with amp mix + UDIs Plate B and Standard Hyb with amp mix, 12 reactions | 96 |
103901 | Twist MF Library Prep + UDIs Plate C and Fast Hyb, 12 reactions | 96 |
104196 | Twist MF Library Prep with amp mix + UDIs Plate C and Fast Hyb with amp mix, 12 reactions | 96 |
103902 | Twist MF Library Prep + UDIs Plate C and Standard Hyb, 12 reactions | 96 |
104197 | Twist MF Library Prep with amp mix + UDIs Plate C and Standard Hyb with amp mix, 12 reactions | 96 |
103905 | Twist MF Library Prep + UDIs Plate D and Fast Hyb, 12 reactions | 96 |
104200 | Twist MF Library Prep with amp mix + UDIs Plate D and Fast Hyb with amp mix, 12 reactions | 96 |
103906 | Twist MF Library Prep + UDIs Plate D and Standard Hyb, 12 reactions | 96 |
104201 | Twist MF Library Prep with amp mix+ UDIs Plate D and Standard Hyb with amp mix, 12 reactions | 96 |
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